Isolation of Thiomolybdate Compounds from the Molybdenum-Iron Protein of Clostridial Nitrogenase
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چکیده
منابع مشابه
Isolation of a molybdenum--iron cluster from nitrogenase.
A molybdenum-iron cluster (Mo-Fe cluster) containing 6 Fe atoms per Mo was isolated by methyl ethyl ketone extraction of component I of nitrogenase from Azotobacter vinelandii. The cluster has no EPR signal in the g = 4 region but has an intense signal at g = 2.05 and 2.01. After the cluster was transferred from methyl ethyl ketone to N-methylformamide, the signal in the g = 2 region disappeare...
متن کاملBiochemistry Metal and sulfur composition of iron - molybdenum cofactor of nitrogenase ( molybdenum - iron protein / dinitrogen reduction / iron - sulfur clusters )
The sulfur content of N-methylformamide solutions of cofactor from Clostridium pasteurianum nitrogenase has been determined to be 11.9 (+0.9) mol per mol of molybdenum. This number was determined radiochemically, using iron-molybdenum cofactor isolated from molybdenum-iron protein from bacteria grown on 35SO4. A total of 3.2 (±0.2) mol of sulfur per mol of molybdenum was found to be present in ...
متن کاملPurification and characterization of the molybdenum-iron protein component of nitrogenase from soybean nodule bacteroids.
The MO-Fe protein from bacteroids of soybean nodules has been purified to a high degree of homogeneity as judged by disc gel electrophoresis and ultracentrifugal analysis. The purification procedure included: polypropylene glycol fractionation, heat treatment, and chromatography on DEAEcellulose and Sephadex G-ZOO. Specific activities of several preparations of the purified protein ranged betwe...
متن کاملIron-molybdenum cofactor biosynthesis in Azotobacter vinelandii requires the iron protein of nitrogenase.
Nitrogenase is composed of two separately purified proteins called the Fe protein and the MoFe protein. In Azotobacter vinelandii the genes encoding these structural components are clustered and ordered: nifH (Fe protein)-nifD (MoFe protein alpha subunit)-nifK (MoFe protein beta subunit). The MoFe protein contains an ironmolybdenum cofactor (FeMo cofactor) whose biosynthesis involves the partic...
متن کاملThe molybdenum--iron protein of Klebsiella pneumoniae nitrogenase. Evidence for non-identical subunits from peptide 'mapping'.
The molybdenum- and iron-containing protein components of nitrogenase purified from Klebsiella pneumoniae, Azotobacter vinelandii, Azotobacter chroococcum and Rhizobium japonicum bacteroids all gave either one or two protein-staining bands after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, depending on the commercial brand of sodium dodecyl sulphate used. The single band obtained...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1978
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1978.tb12686.x